UJI AKTIVITAS ANTIBAKTERI DAN PENGHAMBATAN ENZIM PROTEASE EKSTRAK ETIL ASETAT BATANG Jatropha multifida Linn TERHADAP Staphylococcus aureus ATCC 25923 DAN MRSA
Abstract
The use of antibiotics for the treatment of bacterial infections among people
in many developing drug-resistance. To cope with this, Jatropha multifida Linn can
be used as an alternative for the treatment of bacterial infections because it is known
to have the antibacterial ability. Bacterial protease enzyme inhibition by Jatropha
multifida Linn is expected to reduce the severity or virulence of a bacterial infection.
This study aims to determine the antibacterial activity and protease enzyme
inhibition assay of extract ethyl acetate stem of Jatropha multifida Linn against
Staphylococcus aureus ATCC 25923 and MRSA. Extraction method with a
multilevel soxhletation with solvent n-hexane and ethyl acetate. Antibacterial
activity test to see MIC and MBC using microdilution method. Test of enzyme
inhibition by the method of the wells in a skim milk-MHA 10% with a view
diameter clear zone formed. The results of microdilution MIC and MBC
respectively 0.125 mg / 100μL and 0.25 mg / 100μL for Staphylococcus aureus
ATCC 25923 and MRSA. The presence of the protease enzyme inhibitory activity
is indicated by the formation of a clear zone at a concentration of ½ MIC for S.
aureus and MRSA
Collections
- Pharmacy [1444]