UJI SITOTOKSIK FRAKSI DARI EKSTRAK METANOL RUMPUT GONG (Eriocaulon cinereum R.Br) pada SEL KANKER SERVIKS dan SEL NORMAL (VERO)
Abstract
Eriocaulon cinereum R. Br., known as rumput gong, has been used by the
people in Bangka Belitung as traditional medicine to prevent uncontrolled
cell growth. Preliminary study reported that the ethanol extract of E.
cinereum was able to inhibit cell division of Hela cervical cancer with IC50
value of 427.79 μg/ml. This showed that this herb is potential to be
developed as anticancer. A further study was to evaluate the cytotoxic
activity of the fractions from the methanol extract of E. cinereum on Hela
cell. The aim of this study was to examine the cytotoxic activity of fraction
extract methanol of Rumput Gong (Eriocaulon cinereum R. Br.) against
cervical cancer cells (HeLa) and normal cells (Vero). E. cinereum was
extracted by ultrasound assisted maceration using n-hexane followed by
ethyl acetate and then methanol. The extract was subjected by using
separation tunnel method to obtain dichloromethane, chloroform and water
fractions. The cytotoxic activity was evaluation by MTT assay method, the
absorbance value was obtained using ELISA reader with the wavelength of
595 nm. Due to the amount of the chloroform fraction, no activities
evaluation was carried out. The data were analyzed using PROBIT method
from SPSS 16 for Windows®. The fraction of dichlorometane from
methanol extract of E. cinereum showed cytotoxic activity with IC50 value
of 235.652μg/mL. The water fraction has no cytotoxic activity due because
its IC50 value was 2325.302μg/mL, selectivity index value of fraction
dichlorometan was 2.09 and water fraction was 2.13. The dicholorometane
fraction from methanol extract of E. cinereum has cytotoxic activity against
Hela cell.
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- Pharmacy [1444]