| dc.description.abstract | Latar Belakang: Sodium nitrit merupakan zat aditif yang sering digunakan oleh masyarakat dalam kebutuhan pangan. Konsumsi sodium nitrit berlebihan dapat menurunkan kemampuan hemoglobin dalam mengikat oksigen, sehingga dapat memicu hipoksia jaringan. Sel granular girus dentatus sangat sensitif terhadap hipoksia, beresiko terjadinya apoptosis. Propolis sebagai neuroprotektan yang kandungannya tinggi antioksidan seperti flavonoid, fenol, dan Caffeic Acid Phenethyl Ester (CAPE).
Tujuan Penelitian: Untuk mengetahui pengaruh propolis terhadap jumlah neuron granular di girus dentatus tikus (Rattus novergicus) yang diinduksi sodium nitrit.
Metode Penelitian: Metode ekperimental Post-test with control group. Subjeknya menggunakan tikus jantan (Rattus novergicus) galur wistar, dibagi menjadi 3 kelompok. pertama kelompok kontrol (K) diberikan sodium nitrit 50 mg/kgBB/hari. Kedua, kelompok perlakuan 1 (P1) diberikan sodium nitrit 50 mg/kgBB dengan propolis dosis 100 mg/kgBB /hari. Ketiga, kelompok perlakuan 2 (P2) diberikan perlakuan yang sama namun dengan dosis propolis 200 mg/kgBB/ hari, perlakuan selama 60 hari. Sediaan preparat histologis blok paraffin dengan pewarnaan Hematoksilin-Eosin. Analisis menggunakan uji non parametric Kruskal-Wallis dan dilanjutkan dengan uji Mann-Whitney.
Hasil: Uji non parametric Kruskal-Wallis menunjukkan bahwa terdapat perbedaan jumlah sel granular dari keseluruhan kelompok, p-value=0.004 dengan α<0.05. Uji Mann-Whitney menunjukkan perbedaan jumlah sel granuler yang signifikan antara kelompok kontrol (K) & perlakuan 1 (P1), yaitu p-value=0.006 dan kelompok kontrol (K) & perlakuan 2 (P2) dengan p-value=0.004, sedangkan kelompok perlakuan 1 (P1) & kelompok perlakuan 2 (P2) tidak memberikan pengaruh yang signifikan karena p-value=0.522.
Kesimpulan: Propolis dapat mencegah kerusakan sel neuron granular girus dentatus tikus (Rattus novergicus) dalam kondisi hipoksia.
Background: Sodium nitrite is an additive that is often used by people in food needs. Excessive consumption of sodium nitrite can decrease the ability of hemoglobin in binding oxygen, which can lead to tissue hypoxia. Dentatus granular gene cells are very sensitive to hypoxia, at risk of apoptosis. Propolis as a neuroprotectant with high antioxidant content such as flavonoids, phenols, and Caffeic Acid Phenethyl Ester (CAPE).
Research Objectives: To determine the effect of propolis on the number of granular neurons in the rat dentatus gene (Rattus novergicus) induced sodium nitrite.
Research Methods: Experimental method which uses the Post-test with control group design. The subject of this research is male rat (Rattus novergicus) wistar strain and are divided into 3 groups. The first control group (K) are given sodium nitrite 50mg/kilogram body weight/day. Second group, the treatment group 1 (P1) is given sodium nitrite 50 mg/kilogram body weight with propolis with dose of 100 mg/kilogram body weight/day. Third group, the treatment group 2 (P2) are given the same treatment done to the second group but with a dose of 200 mg of propolis/kilogram body weight/day, the treatment carried out for 60 days. Histological preparations are made using the method of paraffin blocks which then uses Hematoxylin-Eosin for coloring. The results of data observation is done by using non parametric test of Kruskal-Wallis test and Mann-Whitney.
Results: Non parametric test of Kruskal-Wallis suggests that there is a significant difference on the amount of granular cells of the third group of samples, with p-value= 0.004. Test MannWhitney shows the significant difference on the number of granular cells between the control group (K) and treatment 1 (P1) and α<0.05, p-value= 0.006 and control group (K) and treatment 2 (P2) with p-value= 0.004, while treatment group 1 (P1) and treatment group 2 (P2) with statistical tests do not provide a significant influence due to the obtained results of α>0.05, p-value= 0522.
Conclusion: The administration of propolis can maintain and slow the onset of granular neurons cell damage in the dentatus gyrus of rats (Rattus novergicus) induced with sodium nitrite. | en_US |
