| dc.description.abstract | Background: Stroke is one of the highest cardiovascular diseases in the world. Rat stroke model is needed to improve the development of stroke handling. This technique applies Bilateral Common Carotid Artery Occlusion (BCCAO) so it can be known the number of neurons after occlusion and reperfusion compared with normal neurons in the prefrontal cortex of mice.
Objective: Determine the effect of Bilateral Common Carotid Artery Occlusion duration to the number of Rattus norvegicus neuron prefrontal cortex post 24 hours reperfusion.
Methods: The research is experimental research. This research design uses posttest control group design. A total of 20 slide are from 4 groups. Group 1 was the sham operated group, group 2, 3, and 4 were the BCCAO group for 5 minutes, 10 minutes and 20 minutes with 24 hour reperfusion. Data analysis used One Way ANOVA and Post Hoc Tamhane test.
Results: There was a significant difference in the number of prefrontal cortex neurons between the sham operated group and the 10 minute BCCAO duration group, as well as the sham operated and BCCAO duration group of 20 min with p= 0,000 (Confidence Interval = 95%).
Conclusion: There is influence effect of Bilateral Common Carotid Artery Occlusion duration to the number of Rattus norvegicus neuron prefrontal cortex post 24 hours reperfusion.
Latar Belakang: Stroke merupakan salah satu penyakit kardiovaskular yang tertinggi di dunia. Rat stroke model dibutuhkan untuk meningkatkan perkembangan terhadap penanganan stroke. Teknik ini menerapkan Bilateral Common Carotid Artery Occlusion (BCCAO) sehingga dapat diketahui jumlah neuron setelah mengalami oklusi dan reperfusi dibandingkan dengan neuron normal pada korteks prefrontal tikus.
Tujuan Penelitian: Mengetahui pengaruh durasi Bilateral Common Carotid Artery Occlusion terhadap jumlah neuron korteks prefrontalis Rattus norvegicus pasca reperfusi 24 jam.
Metode Penelitian: Penelitian yang dilakukan adalah penelitian eksperimental. Desain penelitian ini menggunakan posttest control group design. Sejumlah 20 preparat berasal dari 4 kelompok. Kelompok 1 adalah kelompok sham operated, kelompok 2, 3, dan 4 adalah kelompok yang dilakukan BCCAO selama 5 menit, 10 menit, dan 20 menit dengan reperfusi 24 jam. Analisis data menggunakan uji One Way ANOVA dan Post Hoc Tamhane.
Hasil: Terdapat perbedaan bermakna jumlah neuron korteks prefrontalis antara kelompok sham operated dan kelompok durasi BCCAO 10 menit, serta kelompok sham operated dan kelompok durasi BCCAO 20 menit dengan nilai p=0,000 (Confidence Interval=95%).
Kesimpulan: Terdapat pengaruh durasi Bilateral Common Carotid Artery Occlusion terhadap jumlah neuron korteks prefrontalis Rattus norvegicus pasca reperfusi 24 jam. | en_US |
