PENGARUH PEMBERIAN PROPOLIS TERHADAP JUMLAH SEL PIRAMIDAL PADA AREA CA1 HIPPOCAMPUS TIKUS (Rattus norvegicus) YANG DIINDUKSI SODIUM NITRIT
Abstract
Background : Sodium nitrite is a food preservative which can cause hypoxia in the tissues if it is consumed in high amounts. Pyramidal neurons of CA1 hippocampus are susceptible region to hypoxia. Neuroprotective agents are required to prevent cell death marked by decreasing number of pyramidal neuron in CA1 hippocampus region. Propolis or bee glue is a resinous product that has many active constituents act as antioxidant and be able to give neuroprotective effect.
Objective : This study aimed to the effect of giving propolis on the number of the pyramidal neuron in CA1 Hippocampus region of rat (Rattus novergicus) induced by sodium nitrite.
Methods : This is an experimental study with post-test control group design. The subjects were 18 adult rats (Rattus norvegicus) Wistar that fulfilled inclusion and exclusion criteria. Subjects were divided into three groups, the control group induced by sodium nitrite, the treatment group 1 (P1) induced by sodium nitrite and given propolis 100 mg/kgBB/day, and the treatment group 2 (P2) induced by sodium nitrite and given propolis 200 mg/kgBB/day. The treatment performed for 60 days. The differences in the mean number of neurons on the entire field of view were analyzed using one-way ANOVA and post-hoc test.
Result : The result of this study showed that there is a significant difference of pyramidal neuron number in CA1 Hippocampus between control group, (P1), and (P2) (P=0,000). Pyramidal neuron numbers of CA1 Hippocampus region in P1 and P2 are higher than those in control group.
Conclusion : The given of Propolis inhibits the reduction pyramidal neuron number in rat’s CA1 Hippocampus region induced by sodium nitrite.
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