| dc.description.abstract | Beside of high nutrien food soybeans contain active compounds of
medicinal values like isoflavones.
Isolation and characterization of isoflavone compound from root of
soybean have been carried out. The isolation by extraction method, and TLC
(Thin Layer Chromatography) have been done.
Identification structure of compounds have been carried out by using ultravioletvisible
spectrophotometer using shift reagents NaOH, A1C1₃ and NaOAc.
The addition of NaOH solution caused bathochromic shift to methanol
spectrum about 18 nm, showed existing of 5-OH and 4'-OH group. The addition
of AlCl₃ gave bathochromic shift about 8 nm, showed existing of 5-OH group and
ketones, and the addition of NaOAc was not gave bathochromic shift, that was not
showed existing of 7-OH group, or C7 at A ring in the shape cf glicosides
isoflavones.
Genistein 7-O-glikocides showed maximum wavelength while at 261 ran,
and compound of fraction 1 absorbed at 271.5 nm Datas above have identified
that compound in fraction 1 is genistein 7-O-glikosida | en_US |
