Perbandingan Kadar Protein pada ASISegar dan ASI yang Disimpan dalam Lemari Es dengan Metode Kjeldahl
Abstract
At the period of this time the press out mother's milk (ASI) saved in the
refrigator represent the best solution to laboring mother's to be they earn remain to
give the exclusively mother's milk for their baby. Exclusive mother's milk represent
the supported of optimalization growth of intelegence of baby brain, because
mother's milk containing all vitamins of required by baby. So a research about
difference of protein in fresh mother's milk which is saved in the refrigator has been
done to know the protein rate in a fresh mother's milk which is saved in the refrigator
difference have of a meaning.
This research is a quantitative analysis done by using Kjeldahl method to
determine the number of nitrogen in the mother's milk. There's only 10 mL of
sample taken for the research. Then it was analyzed by using Kjeldahl method
through destruction phase and using strong acid with K2S04 and HgO functions as
catalyst which can speed up the reaction (chemical process). The destruction resulted
clear solution which is distillated afterward by using reverted cooler. Next it is
titrated by using alcalist. After thatthegrade of nitrogen determined in every sample.
The result of analysis indicates that fresh mother's milk consists of protein as
well as in mother's milk saved in the refrigator. The result of protein rate from fresh
mother's milk and mother's milk saved in refrigator in % b/v is 1,69±0,01 (Fresh
mother's milk); l,61±0,03(Day first of mother's milk); 1,57±0,02(Day second of
mother's milk); 1,57±0,03(Day third of mother's milk); l,56±0,01(Day four of
mother's milk); 1,54±0,02(Day five of mother's milk); l,54±0,02(Day six of
mother's milk); 1,42±0,02(Day seven of mother's milk). The result of one-way
ANOVA statistical experiment indicates that the grade of protein between fresh
mother's milk and that is stored in refrigator has significant difference. The longer it
is stored in the refrigator, the more lower protein contents it will have.
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