VALIDASI METODE ANALISIS KADAR KAFEIN PADA FORMULASI NANOPARTIKEL MENGGUNAKAN METODE KROMATOGRAFI CAIR KINERJA TINGGI (KCKT) DETEKTOR UV
Abstract
Caffeine is a chemical substance which affects in the growth of hair. To optimize the
caffeine activity as the hair grower, an inovation is made by creating the nanoparticle forms of
caffeine. This study aims to perform the validation of method in analyzing caffeine levels on the
nanoparticle forms using HPLC UV detector method. Validation of methods that is used to
analyze caffeine the caffeine levels on the nanoparticle forms is the HPLC method, in the
stationary phase of coloumn C18, the mobile phase of phosphate buffer:acetonitrile(90:10), with
flow rate of 1,2 mL/minute, and the detector with wavelength of 272 nm. The test parameters
done in this study are liniearity test, selectivity test, accuration test and also precision test. The
results of this study show that the method used by the writer have ideal selectivity; the linearity
is also ideal which indicates the r = 0,9997 ; the ideal precision with its RSD values of = 0,37%
and 0,205% ; and the accuration shows the ideal recovery value % ranging from 80-115% is
90,3-94,7%, the LoD value of 2,51 ppm, and LoQ value of 7,62 ppm. In conclusion, the method
in this study is consider valid due to the value parameter validations done in this study suits the
value set by AOAC.
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