Antibiofilm Test of Ethyl Asetate Extract of Jarak Tintir Stem Bark (Jatropha multifida L.) Against Pseudomonas aeruginosa

Abstract

Biofilm is defined as colony of microorganism in the extracellular polymeric substance that produced by bacteria. Pseudomonas aeruginosa is a pathogen bacteria that causing acute and chronic infections. Biofilm of Pseudomonas aeruginosa is difficult to be cured because of their resistance against antibiotics. Ethyl acetate extract of Jatropha multifida L. stem bark that contains alkaloids, flavonoids, and phenols are expected as an antibacterial agent. Previous study showed that the ethyl acetate extract of Jatropha multifida L. stem bark has antibiofilm activity against Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus (MRSA) with IC50 values of 300 μg / mL and 760 μg / mL, respectively. The aim of this study was to examine the inhibition and destruction of ethyl acetate extract of Jatropha multifida L. stem bark against biofilm of Pseudomonas aeruginosa. Antibiofilm activity test was performed using crystal violet microtiter plate assay method. The results showed that ethyl acetate extract of Jatropha multifida L. stem bark has biofilm inhibition percentage of 84.90% and biofilm destruction percentage of 75.86% against Pseudomonas aeruginosa at a concentration of 31.25 μg / mL. Ethyl acetate extract of jarak tintir stem bark (Jatropha multifida L.) has antibiofilm activity against Pseudomonas aeruginosa.