In Vivo Wound Healing Activity of Ethyl Acetate and n-Butanol Fraction of Ampelociscus rubiginosa Lauterb. Tuberous Root in Incisional Wound Model Rats

Abstract

Previous research showed the wound healing activity of ethanol extract of tuberous root of Ampelociscus rubiginosa Lauterb. on incision model rat. This study aims to determine the activity of ethyl acetate (EAF) and n-butanol fraction (NBF) of ethanol extract of A. rubiginosa tuberous root in gel dosage form as wound healing agents. The experimental animals used were rats with incision wound models. Three concentrations of EAF and NBF (0.75%, 1.00%, and 1.25%) were given topically as gel preparations. Bioplacenton was used as a reference. On the 16th days, the length of wound area, tensile strength, and histopathology of skin tissue were determined. The results showed that percent wound contraction was observed significantly greater in EAF and NBF for all concentrations groups (p <0.05). Tensile strength has varied results, but in general both fractions have higher tensile strength value than negative control groups. Histopathological observations showed improvement in re-epithelialization, neocapillarization, and fibroblast. In conclusion, this study suggested that both ethyl acetate and n-butanol fraction of tuberous root of A. rubiginosa have potential benefit as wound healing agents with comparable activity.