| dc.description.abstract | Resistance of malaria parasites towards existing drugs is one of the
causes malaria is still a health problem in the world, include Indonesia, so the
discovery of new antimalarial drugs is required. Nature resources of Indonesia
give a chance to evolve novel medicines from nature materials, one of them is
temu mangga (Curcuma mangga Val.). This study aimed to find out the activities
of antimalarial through the mechanism of hem polymerization inhibition from
ethanol extract and n-hexane:ethyl acetate (2:1) fraction of temu mangga and to
identify compound class that contain within it. The extract of temu manga was
obtained by soxhlet extraction method using 100 % ethanol solvent followed by
fractionation using Vacuum Liquid Chromatography with solvent sequence nhexane,
n-hexane: ethyl acetate (2:1), ethyl acetate and ethanol. This study only
used ethanol extract and n-hexane:ethyl acetate (2:1) fraction to be evaluated for
the activities of hem polymerization inhibition with contain dose 5 mg/mL
compared to positive control (chloroquine) and negative control (DMSO 10%).
This assay performed as many as 3 replications using 5 different concentrations.
Activities of hem polymerization inhibition showed by IC50 values which were
obtained from analysis of relationship between concentration sample and the
percentage of inhibition using the PROBIT on statistical software. The result
showed that ethanol extract and n-hexane:ethyl acetate (2:1) fraction has a mean
IC50 values of 1.67 mg/mL ± 0.83 and 0.73 mg/mL ± 0.17, respectively. Both have
lower IC50 values than chloroquine which has IC50 value of 12 mg/mL, so it can
be said that both have inhibitory activity of hem polimeryzation. The result of
phytochemical screening proved that ethanol extract and n-hexane:ethyl acetate
(2:1) fraction contains terpenoid and phenolic compounds. The dominant
compounds contained in ethanol extract and n-hexane:ethyl acetate (2:1) fraction
based on the identification results of GC-MS was a terpenoid compound, (E)-
labda-8(17),12-dien-15,16-dial, which has retention time of ± 43.49 minutes and
molecular weight of 302 m/z. | en_US |
