| dc.description.abstract | Background: Meniran (Phyllanthus niruri) has pharmacological activity as an
immunostimulant, which can activate neutrophils and lymphocytes. However, the
extract has poor solubility and has impact on both its bioavailability and
pharmacological activity. A self-nano-emulsifying drug delivery system
(SNEDDS) dosage form is made to solve this issue.
Objective: To examine the effect of immunostimulatory activity of the SNEDDS
of meniran based on the number of lymphocytes and neutrophils in male Wistar rats
induced by Staphylococcus aureus bacteria.
Methods: This study used 25 test animals which were divided into 5 test groups
with different treatment; (1) the normal group with distilled water, (2) the negative
group with bases of SNEDDS, (3) the positive group was treated with levamisole
drug, (4) the SNEDDS group with SNEDDS of meniran, and (5) and extract group
with meniran extract preparation. The test animals were treated for 13 days, and
Saureus induction was carried out on the 14th day. Blood samples were taken on
days 0, 7, 14, and 15, and the number of lymphocytes and neutrophils was measured
using a hematology analyzer. The data were presented as mean SD and analyzed
using the ANOVA test (p<0.05).
Result: SNEDDS of meniran increased neutrophil value in rats induced by
Staphylococcus aureus of around 0.40 x 10/ µL but decreased lymphocyte value
of around 3.51 x 10/ µL. On the other hand, meniran extract increased neutrophil
value of around 4.10 x 10/ µL. but decreased lymphocyte value of around 0.65 x
10/ µL.There was no significant difference in lymphocyte and neutrophil values
between SNEDDS of meniran and meniran extract. Compared to the other groups
with p-0.05, they also showed no difference.
Conclusion: SNEDDS of meniran has no activity as an immunostimulant on rats
induced by Staphylococcus aureus. | en_US |
