Penelusuran Hubungan Aktivitas Antioksidan dengan Kadar Flavonoid Total pada Beberapa Ekstrak Etanolik Buah Pare Hijau (Momordica Charantia L.)

Abstract

Bitter melon (Momordica charantia L.) is common and familiar plant widely utilized for food and medicine. Research of Horax, 2005 shows that bitter melon extract contains flavonoids are potent as antioxidants. Some flavonoid known have potent antifree radicals or antioxidants. This study aims to determine the antioxidant activity from bitter melon extract using variation concentration of ethanol in solvent and to know the relationship between levels of total flavonoids with antioxidant activity. Determination of antioxidant activity was evaluated from the scavenging free radical DPPH (2,2- diphenyl-1-pikrilhidrazil) spectrophotometrically. This study used variation of solvent is ethanol 50%, 70%, and 90% in order to determine antioxidant activity of each concentration and vitamin C as comparison, Increased concentrations of extract and vitamin C resulted in the reduction of free radicals are also getting bigger. The value reduction of free radical DPPH was calculated based on it absorbance, measured at λ 517,6 nm. From linear regression result show that IC50 of 50 % ethanol extract are 24,25 ppm, 70% ethanol extract are 43,02 ppm, 90% ethanol extract are 49,94 ppm, and vitamin C (comparison) are 69,54 ppm. These results showed that anti radical activity ethanol extract of bitter melon is higher than vitamin C. The bigger total flavonoids present by 50 % ethanol extract. IC50 value is inversely proportional with total flavonoids, the high flavonoid content is more potential as an antioxidant therefore concentration required to eliminate 50 % of scavenging free radicals activity more smaller. The correlation of total flavonoid with antioxidant activity is directly proportional and inversely proportional to the IC50