Identifikasi Flavonoid Dari Ekstrak Metanol Buah Mahkota Dewa (Phaleria macrocarpa Boerl) Menggunakan Spektrofotometer UV-Vis
Abstract
Mahkota dewa (Phaleria macrocarpa Boerl) is one of the plants of drug
which has been found special quality to treats various diseases. Mahkota dewa
contains active compounds from flavonoids, terpenoids, alkaloids and
polyphenols groups, however the structure of active compounds in methanol
extract have not identified yet.
Identification of flavonoids from the flesh of mahkota dewa has been
conducted. Isolation and separation has been done by extraction method and thin
layer chromatography. Preface identification has been made by thin layer
chromatography using eluen of n-hexane, ethyl acetate, methanol, n-buthanol
acetic acid and water. Authentication purity of flavonoids is made by twodimensional
thin layer chromatography. Separation of flavonoids using
preparative thin layer chromatography. Fraction has been obtained were dissolved
in methanol and were measured its spectrum at wave-length 200 to 400 nm by
UV-Vis spectrophotometer using shift reagents, NaOH, A1C13, HC1, NaOAc and
H3B03.
Preface identification using thin layer chromatography be able to showed
4 fraction with Rr = 78.75, 70.00, 56.25, and 50.00. The best eluen in
development using thin layer chromatography is n-buthanol : acetic acid •water
9.2:6 (v/v). Identification using thin layer chromatography and UV-Vis'
spectrophotometer have been given aresult that the first fraction (R, - 78 75) and
the second fraction (Rf=70.00) contained flavonoids. The result of its spectrum
interpretation, flavonoids which be able to suggested in fraction 1 is flavanon
with 5,7,3, and 4-OH groups (5,7,3',4'-tetrahydroxy flavanon). Flavonoid in
fraction 2is flavonol with ortho-diOH groups at 6,7 or 7,8 ; 3' and 4' -OH groups
; and there is oxygenation at >or 8.
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