UJI AKTIVITAS ANTIDIABETES NANOPARTIKEL PERAK EKSTRAK ETANOL DAN NANOPARTIKEL PERAK EKSTRAK AIR KEMBANG TELANG (Clitoria ternatea L.) SEBAGAI INHIBITOR ENZIM α-GLUKOSIDASE
Abstract
Diabetes mellitus is a chronic diseases which is increasing in Indonesia. To
minimize the side effects of antidiabetic drugs, natural materials such as Kembang
Telang (Clitoria ternatea L.) petals are used as antidiabetic potential. Silver
nanoparticles have been shown to have antidiabetic effects. This study aims to
examine the making of silver nanoparticles of ethanol extract and water extract of
Kembang Telang compared with the extract on the inhibitory activity of the αglucosidase
enzyme.
In
this
research,
it
starts
from
the
making
of
ethanol
extract
Kembang
Telang with infundation modification method and water extract
Kembang Telang using infundation method, biosynthesis of silver nanoparticles
with bottom-up method, identification and characterization by visual observation,
observation of surface resonance of plasmon using UV-Vis spectrophotometer,
particle measurement using Particle Size Analyzer. Then the α-glucosidase
inhibition activity was read absorbance using a UV-Vis spectrophotometer,
continued the calculation of the inhibitory percentage. Percent of data were
analyzed using oneway ANOVA with a confidence level of 95%. The results
showed that silver nanoparticles of ethanol extract and water extract of Kembang
Telang had wavelength, absorbance, particle size and index polydispersity
depending on the range. Inhibition power, the α-glucosidase enzyme is determined
from persent inhibition. From the calculation of percent inhibition obtained values
of 70.44% for ethanol extract silver nanoparticles, 69.37% for water extract silver
nanoparticles, 15.74% for ethanol extracts, 13.56% for water extracts, and 54.17%
for Acarbose. ANOVA results showed a significant difference between silver
nanoparticles with acarbose with a p-value<0.05 and also not significantly different
between nanoparticle of ethanol extract and anoparticle of water extract with a p
value>0.05. It can be concluded that silver nanoparticles of ethanol extract and
water extract Kembang Telang have better inhibition than the extract and the
difference in the solvent used does not affect the inhibition of the α-glucosidase
enzyme.
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- Pharmacy [1444]