Effect of Variations of Solvent Concentration to Antioxidant Activity of Ethanolic Extract of Buas-Buas Stem (Premna Serratifolia L.) Using Dpph (2,2-Diphenyl-1 Picrylhidrazyl) Scavenging Method

Abstract

Buas-buas plant (Premna serratifolia L.) was one of the plants found in Indonesia and was thought to have antioxidant compounds. Buas-buas leaves were reported to contain compounds as antioxidants, in addition to leaves, stems and roots also have compounds as antioxidants. This study aims to determine the effect of ethanol concentration in the ethanolic extract of buas-buas stem as antioxidants by DPPH scavenging method. Ethanol was used as solvent with concentration of 70%, 80%, 90%, and 96%. The extraction was done by Soxhlet extraction method, where the extract obtained was determined for the total phenolic content by using Folin-Ciocalteu method, and antioxidant activity by DPPH scavenging method. The results showed that the highest total phenolic content of 90% ethanol extract was 56.4% equivalent of gallic acid (EAG). Based on the % inhibition value analyzed by using SPSS 21 with Moses Extreme Reactions test obtained the p value <0.05, where the concentration of solvent can affect the antioxidant activity of ethanolic extract of buas-buas stem. While the antioxidant activity that has the highest IC50 value was shown by 80% ethanol extract with a value of 63.93 mg / L.