http://hdl.handle.net/123456789/3480 2026-04-25T17:01:23Z http://hdl.handle.net/123456789/6253 PERBANDINGAN PEMBUATAN SEDIAAN GEL ANTISEPTIK MINYAK ATSIRI SIRIH MERAH (Piper crocatum) DENGAN VARIASI KONSENTRASI HIDROKSI PROPIL METIL SELULOSA (HPMC) DAN NATRIUM KARBOKSIMETIL SELULOSA (NA CMC) Juliantina Rachmawaty, Farida; Devi Miswida, Chintya; Triastuti, Asih; Arum Sari, Desy Mencuci tangan merupakan hal sederhana namun sangat penting dalam perilaku hidup bersih dan sehat, serta dapat mencegah sebagian besar penyakit infeksi. Saat ini dengan alasan praktis telah umum digunakan gel antiseptik yang dapat digunakan tanpa air. Umumnya gel antiseptik yang tersedia berbasis alkohol. Namun, banyak kalangan tidak menyukai, dengan alasan membuat kulit kering, iritasi ataupun karena kehati-hatiannya agar makanan yang dimakan tidak tercampur alkohol. Di sisi lain saat ini slogan back to nature semakin marak di masyarakat. Minyak atsiri sirih merah (Piper crocatum) telah terbukti memiliki sifat antibakteri baik terhadap bakteri gram positif maupun gram negatif. Tujuan penelitian ini adalah untuk mengkaji pengaruh variasi eksipien Hidroksi Propilmetil Selulosa (HPMC) dan Natrium Karboksimetil Selulosa (Na CMC) terhadap sifat fisik dan daya antiseptik, demikian juga mengenai aseptabilitas serta menentukan sediaan terbaik antara penggunaan eksipien HPMC dan Na CMC Minyak atsiri daun sirih merah diperoleh dengan cara destilasi uap air. Gel antiseptik masing-masing dibuat dalam 3 formulasi dengan perbedaan variasi konsentrasi basis HPMC 0,75%, 1,50% dan 3,00%, sementara untuk Na CMC 0,5%, 1,0% dan 1,5%. Uji terhadap gel meliputi uji homogenitas dan transparansi, pengukuran pH, uji daya sebar, uji daya lekat dan pengukuran viskositas, daya antiseptik dan uji aseptibilitas. Uji sifat fisik dianalisis secara deskripstif dan uji statistik ANOVA dua arah. Aseptabilitas gel dianalisis secara deskriptif dan uji statistik ANOVA satu arah. Daya antiseptik gel dianalisis dengan uji ANOVA satu arah. Adanya peningkatan konsentrasi HPMC, daya sebar gel semakin kecil sedangkan daya lekat dan viskositas sediaan semakin besar, namun tidak berpengaruh terhadap homogenitas, transparansi dan pH. Aseptabilitas yang paling baik pada konsentrasi tertinggi (3,00%) sedangkan gel dengan konsentrasi HPMC 0,75% memiliki daya antiseptik paling baik. Sementara untuk Na CMC semakin tinggi konsentrasi yang digunakan menyebabkan peningkatan daya lekat, peningkatan viskositas dan penurunan daya sebar gel. Formula gel dengan konsentrasi Na CMC 0,50% berpengaruh terhadap transparansi dan pH gel, sedangkan formula gel dengan konsentrasi Na CMC 1% dan 1,5% tidak berpengaruh. Konsentrasi Na CMC 0,50% memberikan sifat aseptabilitas dan daya antiseptik yang paling baik. Untuk hasil keseluruhan formulasi yang direkomendasikan adalah formulasi gel eksipien HPMC 0,75% dengan perbaikan fisik gel. Collections > Lecturers > Faculty of medicine > Proceeding Scientific Forum 2015-11-11T00:00:00Z http://hdl.handle.net/123456789/6251 Antibacterial Activity Comparison between the Fraction of Ethyl Acetate, Chloroform, and Methanol of Red Betel Vine (Piper crocatum) Leaves Ethanol Extract toward Salmonella typhi Juliantina Rachmawaty, Farida; Anshory Tamhid, Hady; Hidayah, Ridho Background :Salmonella typhiisthe cause oftyphoidfeverdisease. This bacteria was reported to have resistance towards antibiotics at several areas in Indonesia. As an efforttoovercomethis resistance;; it is necessary to find a new drug. Rachmawaty (2009) reported that ethanol red betel leaves extract has antibacterial activity towards. Staphylococcus aureus and Escherichia coli. This research aims to compare antibacterial activity of ethanol red betel vine leaves extract fractions to Salmonella typhi. Method : The extract was made with maceration method using 70% solvent. It is fractionated with column vacuum chromatography method in sequence with n-hexane, ethyl acetate, chloroform, and methanol solvent. The antibacterial activity is performed by serial dilution method and inoculates in Mac Conkey medium. The tested serial concentration was SO%, 25%, 12.5%, and 6.25%. The antibacterial activity of each fraction was compared and the best fraction was identified using Thin Layer Chromatography and phytochemistry screening. Result : This study found that red betel vine leaves extract fraction with the best antibacterial activity was methanol. It had 25% b/v Minimum Bactericidal Concentration (MBC). The identification result showed that methanol fraction contained saponin and flavonoid. Conclusion : The best antibacterial activity toward Salmonella typhi is found in methanol fraction from the ethanol extract of reci betel vine leaves. The compounds obtained in the fraction were flavonoid and saponin. Collections > Lecturers > Faculty of medicine > Proceeding Scientific Forum 2015-10-29T00:00:00Z http://hdl.handle.net/123456789/6250 PERIODONTITIS AND CORONARY HEART DISEASE Juliantina Rachmawaty, Farida Periodontitis is a serious infection or inflammation of the gums that involves the destruction of soft tissues and bones supporting the teeth. The causes of periodontitis are some species of gram-negative bacteria colonizing on : · dental plaque in the subgingival area. Research reports, the disease not only has local effects. Other researchers report that people with the periodontal infection have a higher risk for coronary heart disease (CHD) than those who do not suffer. Research shows that periodontitis sufferers are 1.5-4 tin1es more likely to develop cardiovascular disease. On the other hand, coronary heart disease is the leading cause of death for non-infectious diseases. In periodontal infections there is an increase in C-reactive protein and proinflainn1atory activity, but HDL-cholesterol is lower than control. The presence of such infections affects the endothelial cells, blood coagulation, fat metabolisnl and Inonocytes or macrophages. Poor oral health will increase the incidence of periodontitis infection. Periodontitis increases the risk of coronary heart disease. Collections > Lecturers > Faculty of medicine > Proceeding Scientific Forum 2018-03-23T00:00:00Z http://hdl.handle.net/123456789/6249 BACTERIA THAT CAUSED RHEUMATIC HEART DISEASE Juliantina Rachmawaty, Farida Rheumatic Heart Disease (RHO) is a disease of the heart as a result of residual symptoms (sequelae} of Rheumatic Fever (RF), which marked the occurrence of heart valve defects. Rheumatic fever is an autoimmune disease that attacks the multi system due to infection of Streptococcus group A a-hemolyticus of the pharynx (pharyngitis). These bacteria usually attack children and young adults. Rheumatic fever causes inflammation that usually occurs in the heart, skin and connective tissue. In endemic areas, 3% of patients with pharyngitis by Streptococcus develop rheumatic fever within 2-3 weeks after the infection of the upper respiratory tract. Approximately 40-60% of patients experience acute rheumatic fever that develops into Rheumatic Heart Disease. The bacteria are believed as Streptococcus a-hemolyticus group A. This bacterium is a gram-positive bacteria, cocci shaped with the trademark arranged like a chain on microscopic examination. The length of the chain is highly variable and influenced by environmental factors. The chain will be longer in liquid medium rather than in solid medium. The bacteria can do hemolysis to the blood perfectly so that the seed so that the blood look like a clear area around the colony. Protein M, virulence factors found in the cell walls of Streptococcus group A a-hemolyticus, has similarities with the structural protein in the myocardium, heart valves, synovial, skin and sub thalamic that causes cross-reactions. Collections > Lecturers > Faculty of medicine > Proceeding Scientific Forum 2016-05-14T00:00:00Z