The research on cyclic voltammetry studies of uric acid with Gold working electrode (Au) and its application to the analyze of urine have been done. Cyclic voltammetry analysis method with high sensitivity and easy to implement have been investigated in the analysis of uric acid. Characterization of urea peak in voltamogram was conducted to determine the characteristic specific peak of uric acid. The effect of using electrolyte, uric acid concentration studied and determined to produce the optimum method. Analysis applications such as linearity, limit of detection (LOD), limit of quantification (LOQ) and recovery, through the application studied in urine with the found method. The result showed that the characteristic peaks of uric acid found in the system can be turned Epa 0.513611 V with NaOH 0,1 M electrolyte. Effect of concentration on current showed linearity with R2= 0.997. Application analysis obtain satisfactory results with, R2 = 0.997, LOD=0.00067 M dan LOQ= 0.00223 M with  100.261 % of recovery, and uric acid concentration in the human urine = 4.679 mg/dL. 
Keywords: cyclic voltammetry, uric acid, gold electrode