Determination of uric acid in human urine using cyclic voltammetry by nickel electrode has been carried out. The cyclic voltammetry were performed in a three electrodes system using nickel as a working electrode, an Ag/AgCl (saturated KCl) as a reference electrode and platinum wire as the counter electrode. The voltamogram that conducted from the analysis was used to determine characteristic specific peak of uric acid. Parameters of method validation of uric acid analysis of urine are precision, accuration (recovery), limit of detection (LOD), and limit of quantification (LOQ). Electroanalysis of uric acid was performed in 0,1 M NaOH as an electrolyte. The result of the study showed that the correlation of determination using nickel electrode for electroanalysis uric acid was R2= 0,998. Application analysis obtain satisfactory results with LOD = 0,59 mg/dL and LOQ = 1,98 mg/dL with recovery 100,95 %. And uric acid concentration in the human urine = 2.4025 mg/dL. As a conclution, the methods can be used for routine analysis of a uric acid. Simplicity of sample preparation and use of low cost reagents are the additional benefit of this method. 
Keywords: cyclic voltammetry, uric acid, nickel electrode.